Willem Van Eden. Annika B. Home Contact us Help Free delivery worldwide. Free delivery worldwide. Bestselling Series. Harry Potter. Popular Features. New Releases. Description Antimicrobial peptides have been the subject of intense research in the past decades, and are now considered as an essential part of the defense system in bacteria, plants, animals and humans. The book starts with an overview of the evolution of cysteine-containing antimicrobial peptides including defensins , and the role of these peptides in host defense in plants and micro-organisms.
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The realization that antimicrobial peptides also display functions distinct from their direct antimicrobial action is the focus of the next chapters, and puts these peptides center stage in immunity and wound repair. Further chapters discuss the role of antimicrobial peptides in disease, by providing an overview of mechanisms in bacterial resistance to antimicrobial peptides and a discussion of their role in inflammatory bowel disease, cystic fibrosis lung disease and chronic obstructive pulmonary disease.
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Finally, the book shows how knowledge of the function of antimicrobial peptides and their regulation can be used to design new therapies for inflammatory and infectious disorders. This is a very important area of research because of the increase in resistance of micro-organisms to conventional antibiotics. Therefore the use of synthetic or recombinant peptides, or agents that stimulate the endogenous production of antimicrobial peptides, provides an attractive alternative for conventional antibiotics.
Product details Format Paperback pages Dimensions x x Illustrations note 20 Illustrations, color; 23 Illustrations, black and white; XI, p. Other books in this series. Add to basket. Arachidonate Remodeling and Inflammation Alfred N. Novel Cytokine Inhibitors G. Pain and Neurogenic Inflammation Susan D. Apoptosis and Inflammation James Winkler. Nitric Oxide and Inflammation D. In the mouse term labour is initiated through progesterone withdrawal and is associated with much less inflammation This corresponds with our observation in preliminary experiments examining mCRAMP expression throughout mouse gestation, where we found no evidence of upregulation in term labour.
In humans, cathelicidin protein levels are increased in myometrium undergoing term labour. These data suggest that cathelicidin plays a role in mediating some of the effects which lead to inflammatory PTB in mice, and may also be relevant in the onset of human labour. Following matings, mice were housed in grouped cages of up to 6 mice per cage under specific pathogen free conditions at the same facility. Mice were permitted food and water as required and maintained in a hour cycle of light and dark. All mice were acclimatized for a minimum of 1 week before use.
Female mice were mated with stud males from the same genotype. The presence of a vaginal copulatory plug indicated day 1 of gestation. Pregnant mice with no appearance of a plug were not used in experiments, as gestational age was uncertain. The ultrasound guided intrauterine injection mouse model of PTB previously optimised in our group was used Differences in the inflammatory response were also investigated Fig.
Space between two intra-amniotic sacs was located with ultrasound where the injection was administered. After recovery, mice were monitored using closed circuit television cameras and a video recorder. Time of delivery was the time between injection and delivery of first pup. A separate cohort of mice was used to assess the inflammatory effect of the stimuli used on maternal tissues.
Antimicrobial Peptides and Innate Immunity
These mice were 17 days of gestation and received no treatment, and were culled in the same manner described above. Protein was added to 2. A positive control of 0. The intensity of fluorescence in each lane was calculated using Image Studio Version 4. Myometrium was collected from the inferior margin of the incision site of women delivering by caesarean section, at the Royal Infirmary, Edinburgh.
Labour was defined as regular uterine contractions with cervical dilation. Negative controls were performed using NGS in place of the primary antibody.
Cathelicidins and the Onset of Labour | Scientific Reports
Images at Magnification of x40 were captured on the LSM confocal microscope. At least five areas were imaged from each sample. Four individual patient samples were analysed from the TL group and 5 patient samples were analysed from the TNL group. Image files were then imported into ImageJ and analysed using the Cell Counter plug-in. LL positive cells were counted in each section and expressed as a percentage of total cells in section which was counted as total number of DAPI nuclei in the section.
Saigal, S. An overview of mortality and sequelae of preterm birth from infancy to adulthood. Goldenberg, R. Epidemiology and causes of preterm birth. Kahlenberg, J. Little peptide, big effects: the role of LL in inflammation and autoimmune disease. Beaumont, P. LL an immunomodulatory antimicrobial host defence peptide. In Antimicrobial peptides and Innate Immunity. Progress in Inflammation Research eds Hiemstra, P.
Bowdish, D. Impact of LL on anti-infective immunity.
Yang, D. LL, the neutrophil granule- and epithelial cell-derived cathelicidin, utilizes formyl peptide receptor-like 1 FPRL1 as a receptor to chemoattract human peripheral blood neutrophils, monocytes, and T cells. J Exp Med , — Heilborn, J. The cathelicidin anti-microbial peptide LL is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium.
Koczulla, R. J Clin Invest , — Human cathelicidin, hCAP, is processed to the antimicrobial peptide LL by extracellular cleavage with proteinase 3. Blood 97 , — Frew, L. Antimicrobial peptides and pregnancy. Yarbrough, V. Antimicrobial peptides in the female reproductive tract: a critical component of the mucosal immune barrier with physiological and clinical implications. Gallo, R. Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse.
J Biol Chem , — Cathelicidin host defence peptide augments clearance of pulmonary Pseudomonas aeruginosa infection by its influence on neutrophil function in vivo. Agrawal, V. Intrauterine infection and preterm labor. Cappelletti, M. Inflammation and preterm birth. Christiaens, I. Inflammatory processes in preterm and term parturition. Gomez-Lopez, N. Immune cells in term and preterm labor. Gotsch, F. Migale, R. Modeling hormonal and inflammatory contributions to preterm and term labor using uterine temporal transcriptomics. Osman, I. Leukocyte density and pro-inflammatory cytokine expression in human fetal membranes, decidua, cervix and myometrium before and during labour at term.
Mol Hum Reprod 9 , 41—45 Young, A. Immunolocalization of proinflammatory cytokines in myometrium, cervix, and fetal membranes during human parturition at term. Biol Reprod 66 , — Rinaldi, S. Ultrasound-guided intrauterine injection of lipopolysaccharide as a novel model of preterm birth in the mouse.
Elovitz, M. A new model for inflammation-induced preterm birth: the role of platelet-activating factor and Toll-like receptor Bollapragada, S. Term labor is associated with a core inflammatory response in human fetal membranes, myometrium, and cervix.
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Am J Obstet Gynecol , Larrick, J. Human CAP a novel antimicrobial lipopolysaccharide-binding protein. Infect Immun 63 , — Rosenfeld, Y. Endotoxin lipopolysaccharide neutralization by innate immunity host-defense peptides. Peptide properties and plausible modes of action. M McHugh, B.
Elssner, A. A novel P2X7 receptor activator, the human cathelicidin-derived peptide LL37, induces IL-1 beta processing and release. J Immunol , — Tjabringa, G. Human cathelicidin LL is a chemoattractant for eosinophils and neutrophils that acts via formyl-peptide receptors. Pinheiro da Silva, F. Differing effects of exogenous or endogenous cathelicidin on macrophage toll-like receptor signaling. Severino, P. Cathelicidin-deficient mice exhibit increased survival and upregulation of key inflammatory response genes following cecal ligation and puncture.
Boyle, A. Repurposing simvastatin as a therapy for preterm labor: evidence from preclinical models. Herrera, C. Immune cell and transcriptomic analysis of the human decidua in term and preterm parturition. Robertson, S. Interleukin-6 is an essential determinant of on-time parturition in the mouse. Geiser, T. Moser, B. Ong, P. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. Nakamichi, Y. Roles of cathelicidins in inflammation and bone loss. Horibe, K. Roles of cathelicidin-related antimicrobial peptide in murine osteoclastogenesis.
Kemp, M. Preterm birth, infection, and inflammation advances from the study of animal models. Lim, R. Singh, N. Is myometrial inflammation a cause or a consequence of term human labour? J Immunol Methods , 53—59 Gonzalez, J. Comparison of the mechanisms responsible for cervical remodeling in preterm and term labor. Nizet, V. Innate antimicrobial peptide protects the skin from invasive bacterial infection.
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